![quantification western blot imagej quantification western blot imagej](https://www.spandidos-publications.com/article_images/mmr/8/2/MMR-08-02-0585-g00.jpg)
Use the Straight Line Selection Tool↑ to draw base lines and/or drop lines so that each peak of interest defines a closed area (ImageJ will automatically switch to the Straight Line tool).Select Gels ▷↑Plot Lanes ( 3 ) to generate the lane profile plots.Repeat the previous step for each remaining lane.The selected lane is outlined and labelled, and ‘Lane n selected’ is displayed in the status bar. Move the rectangular selection right to the next lane (or down if the lanes are horizontal) and select Gels ▷↑Select Next Lane ( 2 ).Select Gels ▷↑Select First Lane ( 1 ) and the lane will be outlined and ‘Lane 1 selected’ displayed in the status bar.This should be the left most lane if the lanes are vertical or the top lane if the lanes are horizontal.
![quantification western blot imagej quantification western blot imagej](https://www.frontiersin.org/files/Articles/413877/fmicb-09-02689-HTML/image_m/fmicb-09-02689-g004.jpg)
Use the rectangular selection tool to outline the first lane.Note that a copy of the gel image with the lane outlines can be created at any point using the Image ▷Overlay ▷ Flatten ↑ command. For practice, refer to the video tutorial on the ImageJ wikipage and use the File ▷ Open Samples ▷↑Gel sample image (1-D gel) to perform the following steps.